Services
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Instrumentation and services are available to all researchers inside and outside of the university.
All users are responsible for their own laboratory safety training to be current during each use of the core facilities.
Biophysics core will assist research projects needing macromolecular structure determination and the biophysical characterization of proteins in collaboration using X-ray crystallography (at Argonne) and Cryo-EM (at University of Chicago). Please contact Hyun Lee (danielhl@uic.edu)
Biophysics Core Instruments
Biophysics Core Instruments
| Instrument Name | Manufacturer | Model | Location | Training |
|---|---|---|---|---|
| Biacore T200 | Cytiva (former GE Healthcare) | Biacore T200 | CfSB room 113 | Available |
| Biacore 8K | Cytiva (former GE Healthcare) | Biacore 8K | CfSB room 113 | Available |
| Integra VIAFLO384_96 Pipette Head | Integra | VIAFLO384 | CfSB room 113 | Available |
| Jasco 815 Circular Dichroism (CD) | Jasco | J-815-150S | CfSB room 113 | Available |
| PEAQ-ITC MicroCalorimeter | MicroCal, LLC | PEAQ-ITC | CfSB room 113 | |
| VP-ITC MicroCalorimeter | MicroCal,LLC | VP-ITC | CfSB room 113 | Available |
| FPLC_AKTA Pure | Cytiva (Former GE Healthcare) | AKTA Pure | CfSB room 115 | Available |
| NanoDrop Micro-UV/Vis Spectrophotomenter | Thermo Scientific | NanoDrop One | CfSB room 115 | Available |
| C25KC Incubator shaker | New Brunswick Scientific | C25KC | CfSB room 115 | Available |
| Inova 4200 Incubator shaker | Inova | 4200 | CfSB room 115 | Available |
| Centrifuge_Sorvall LYNX 4000 | Sorvall | Lynx4000 | CfSB room 115 | Available |
| Emulsiflex C5 Homogenizer | Avestin | Emulsiflex C5 | CfSB room 118 | Available |
| Sonic Dismembrator Model500 Sonicator | Thermo Fisher Scientific | Model 500 | CfSB room 118 | Available |
| Fisher Scientific 60 Sonic Dismembrator | Thermo Fisher Scientific | Model 60 | CfSB room 118 | Available |
| PerkinElmer Victor3V plate reader | PerkinElmer | Victor3V 1420-040 | CfSB room 118 | Available |
| C280 Azure Biosystem | Azure Biosystems | C280 | CfSB room 118 | Not Available |
Biophysics Core Services & Prices
Below are internal rates for UIC, University of Chicago, and Northwestern University. External academic and commercial rates are 1.599X and 2X, respectively.
Please note that rates are subject to change.
Instrument rates for self-users
| Instrument Name | Internal UIC rate | Unit |
|---|---|---|
| Biacore T200 | $200.16 | per day |
| Biacore 8K | $225.12 | per day |
| Integra VIAFLO384_96 Pipette Head | $7.50 | 10 min |
| Refeyn TwoMP Mass Photometer | $60.00 | 30min |
| Jasco 815 Circular Dichroism (CD) | $45.00 | 1 h |
| PEAQ-ITC MicroCalorimeter | $45.00 | 1h |
| VP-ITC MicroCalorimeter | $20.00 | 1 h |
| FPLC_AKTA Pure | $27.50 | 1 h |
| NanoDrop Micro-UV/Vis Spectrophotomenter | $3.75 | 5 min |
| C25KC Incubator shaker | $1.00 | 1 h |
| Inova 4200 Incubator shaker | $1.00 | 1 h |
| Centrifuge_Sorvall LYNX 4000 | $22.50 | 30 min |
| Emulsiflex C5 Homogenizer | $22.50 | 30 min |
| Sonic Dismembrator Model500 Sonicator | $22.50 | 30 min |
| Fisher Scientific 60 Sonic Dismembrator | $22.50 | 30 min |
| BMG Biotech Optima plate reader | $22.50 | 30min |
| PerkinElmer Victor3V plate reader | $22.50 | 30 min |
| C280 Azure Biosystem | $7.50 | 10 min |
Biophysics Core Services
Below are internal rates for UIC, University of Chicago, and Northwestern University. External academic and commercial rates are 1.599X and 2X, respectively.
Please note that rates are subject to change.
Biophysics Core Services
| Service Name | Instrument | Staff time | Consumables | Data Analysis | Estimated UIC internal price |
|---|---|---|---|---|---|
| SPR service with Biacore T200 | $200.16/day | 4 h ($50.00/h) | $150.00/day | 1 h ($91.00/h) | $641.16 for 4 analytes in duplicates excluding chip price |
| SPR service with Biacore 8K | $225.12/day | 4 h ($50.00/h) | $230.00/day | 1 h ($91.0/h) | $837.12 for 4 analytes in duplicates excluding chip price |
| MP service with Refeyn TwoMP | $120/h | 1 h ($50/h) | $35/1 h runs | 0.5 h ($91/h) | $226.54 for 2-4 samples |
| CD service | 2 h ($45.00/h) | 1 h ($50.00/h) | $5.00/run | 0.5 h ($91.00/h) | $147.51/sample, |
| ITC service | 7 h ($20.00/h) | 4.5 h ($50.00/h) | $23.00/run | 0.5 h ($91.00/h) | $402.86/sample with buffer control |
| Assay Service (ATPase, Kinase, Protease, and Thermal shift assay) | $45.00/h | $50.00/h | $91.00/h | Price can vary depending on how much staff time & consumables are required | |
| Enzyme Assay_IC50 determination | 0.5 h ($45.00/h) | 4 h ($50.00/h) | $45.56/run | 1 h ($91.00/h) | $375.29 for 4 samples in triplicates, $47.50/additional sample |
Protein over-expression and Purification
Large-scale protein purification service is offered to users who need purified proteins for SPR, High-Throughput Screening (HTS), and structural biology (X-ray crystallography and NMR). AKTA Pure FPLC enables us to purify 2-3 different proteins at a time using a well-optimized two-step (HisTrap and Size-exclusion columns) purification protocol.
Below are internal rates for UIC, University of Chicago, and Northwestern University. External academic and commercial rates for instruments and staff times are 1.599X and 2X, respectively. Consumables are same for all users.
Please note that rates are subject to change.
Protein over-expression and Purification
| Service Name | Estimated UIC internal price | Unit |
|---|---|---|
| Transformation into BL21DE3 or Rosetta2DE3 | $75.00 | 1 plasmid |
| Prepare glycerol stock | $25.00 | 1 plasmid |
| Protein Mini-expression test | $180.00 | 1 protein, $70/additional sample |
| 1-2L over-expression | $200.00 | 1 protein |
| Purification_Cell lysis-Affinity (HisTrap, or GSTtrap) | $700.00 | 1 protein |
| Purification_SEC (16/60 SD75 or SD200 or 26/60 SD200) | $239.00 for 16/60 SD75 or SD200 | 1 protein |
| Tag cleavage & reload | $200.00 | 1 protein |
| Purification_FPLC run_Affinity service | $258.30 | 1 protein |
| Purification_FPLC run_Ion Exchange Service | $270.30 | 1 protein |
Trainings
Below are internal rates for UIC, University of Chicago, and Northwestern University. External academic and commercial rates are 1.599X and 2X, respectively.
Please note that rates are subject to change.
Trainings
| Instrument Name | 1 person | 2 people in a group | 3 people in a group | Unit |
|---|---|---|---|---|
| SPR BiacoreT200 or 8K for new users | $329.27 | $230.49 | $181.10 | 1 person |
| SPR Biacore 8K for T200 users | $209.79 | $146.85 | $115.38 | 1 person |
| Integra VIAFLO384 Pipette Head | $27.30 | $20.48 | $16.68 | 1 person |
| Refeyn TwoMP Mass Photometer | $165.00 | $110.00 | $91.67 | 1 person |
| Jasco 815 Circular Dichroism (CD) | $91.68 | $64.18 | $49.62 | 1 person |
| PEAQ-ITC MicroCalorimeter | $165.00 | $110.00 | $91.67 | 1 person |
| VP-ITC MicroCalorimeter | $240.60 | $168.42 | $132.33 | 1 person |
| FPLC_AKTA Pure | $255.60 | $178.92 | $140.58 | 1 person |
| NanoDrop Micro-UV/Vis Spectrophotomenter | $12.63 | $8.84 | $6.95 | 1 person |
| C25KC Incubator shaker | $9.23 | $6.46 | $5.08 | 1 person |
| Inova 4200 Incubator shaker | $9.23 | $6.46 | $5.08 | 1 person |
| Centrifuge_Sorvall LYNX 4000 | $14.03 | $9.82 | $7.72 | 1 person |
| Emulsiflex C5 Homogenizer | $90.22 | $63.16 | $49.62 | 1 person |
| Sonic Dismembrator Model500 Sonicator | $60.15 | $42.10 | $33.08 | 1 person |
| Fisher Scientific 60 Sonic Dismembrator | $60.15 | $42.10 | $33.08 | 1 person |
| PerkinElmer Victor3V plate reader | $57.65 | $40.35 | $31.71 | 1 person |
Macromolecular Structural Biology
Biophysics core will assist research projects needing macromolecular structure determination and the biophysical characterization of proteins in collaboration using X-ray crystallography (at Argonne) and Cryo-EM (at University of Chicago). Please contact Hyun Lee (danielhl@uic.edu).