Services
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Instrumentation and services are available to all researchers inside and outside of the university.
All users are responsible for their own laboratory safety training to be current during each use of the core facilities.
Biophysics core will assist research projects needing macromolecular structure determination and the biophysical characterization of proteins in collaboration using X-ray crystallography (at Argonne) and Cryo-EM (at University of Chicago). Please contact Hyun Lee (danielhl@uic.edu)
Biophysics Core Instruments Heading link
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Biophysics Core Instruments
Instrument Name Manufacturer Model Location Training Biacore T200 Cytiva (former GE Healthcare) Biacore T200 CfSB room 113 Available Biacore 8K Cytiva (former GE Healthcare) Biacore 8K CfSB room 113 Available Integra VIAFLO384_96 Pipette Head Integra VIAFLO384 CfSB room 113 Available Jasco 815 Circular Dichroism (CD) Jasco J-815-150S CfSB room 113 Available PEAQ-ITC MicroCalorimeter MicroCal, LLC PEAQ-ITC CfSB room 113 VP-ITC MicroCalorimeter MicroCal,LLC VP-ITC CfSB room 113 Available FPLC_AKTA Pure Cytiva (Former GE Healthcare) AKTA Pure CfSB room 115 Available NanoDrop Micro-UV/Vis Spectrophotomenter Thermo Scientific NanoDrop One CfSB room 115 Available C25KC Incubator shaker New Brunswick Scientific C25KC CfSB room 115 Available Inova 4200 Incubator shaker Inova 4200 CfSB room 115 Available Centrifuge_Sorvall LYNX 4000 Sorvall Lynx4000 CfSB room 115 Available Emulsiflex C5 Homogenizer Avestin Emulsiflex C5 CfSB room 118 Available Sonic Dismembrator Model500 Sonicator Thermo Fisher Scientific Model 500 CfSB room 118 Available Fisher Scientific 60 Sonic Dismembrator Thermo Fisher Scientific Model 60 CfSB room 118 Available PerkinElmer Victor3V plate reader PerkinElmer Victor3V 1420-040 CfSB room 118 Available C280 Azure Biosystem Azure Biosystems C280 CfSB room 118 Not Available
Biophysics Core Services & Prices Heading link
Below are internal rates for UIC, University of Chicago, and Northwestern University. External academic and commercial rates are 1.599X and 2X, respectively.
Please note that rates are subject to change.
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Instrument rates for self-users
Instrument Name Internal UIC rate Unit Biacore T200 $200.16 per day Biacore 8K $225.12 per day Integra VIAFLO384_96 Pipette Head $7.50 10 min Refeyn TwoMP Mass Photometer $60.00 30min Jasco 815 Circular Dichroism (CD) $45.00 1 h PEAQ-ITC MicroCalorimeter $45.00 1h VP-ITC MicroCalorimeter $20.00 1 h FPLC_AKTA Pure $27.50 1 h NanoDrop Micro-UV/Vis Spectrophotomenter $3.75 5 min C25KC Incubator shaker $1.00 1 h Inova 4200 Incubator shaker $1.00 1 h Centrifuge_Sorvall LYNX 4000 $22.50 30 min Emulsiflex C5 Homogenizer $22.50 30 min Sonic Dismembrator Model500 Sonicator $22.50 30 min Fisher Scientific 60 Sonic Dismembrator $22.50 30 min BMG Biotech Optima plate reader $22.50 30min PerkinElmer Victor3V plate reader $22.50 30 min C280 Azure Biosystem $7.50 10 min
Biophysics Core Services Heading link
Below are internal rates for UIC, University of Chicago, and Northwestern University. External academic and commercial rates are 1.599X and 2X, respectively.
Please note that rates are subject to change.
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Biophysics Core Services
Service Name Instrument Staff time Consumables Data Analysis Estimated UIC internal price SPR service with Biacore T200 $200.16/day 4 h ($50.00/h) $150.00/day 1 h ($91.00/h) $641.16 for 4 analytes in duplicates excluding chip price SPR service with Biacore 8K $225.12/day 4 h ($50.00/h) $230.00/day 1 h ($91.0/h) $837.12 for 4 analytes in duplicates excluding chip price MP service with Refeyn TwoMP $120/h 1 h ($50/h) $35/1 h runs 0.5 h ($91/h) $226.54 for 2-4 samples CD service 2 h ($45.00/h) 1 h ($50.00/h) $5.00/run 0.5 h ($91.00/h) $147.51/sample, ITC service 7 h ($20.00/h) 4.5 h ($50.00/h) $23.00/run 0.5 h ($91.00/h) $402.86/sample with buffer control Assay Service (ATPase, Kinase, Protease, and Thermal shift assay) $45.00/h $50.00/h $91.00/h Price can vary depending on how much staff time & consumables are required Enzyme Assay_IC50 determination 0.5 h ($45.00/h) 4 h ($50.00/h) $45.56/run 1 h ($91.00/h) $375.29 for 4 samples in triplicates, $47.50/additional sample
Protein over-expression and Purification Heading link
Large-scale protein purification service is offered to users who need purified proteins for SPR, High-Throughput Screening (HTS), and structural biology (X-ray crystallography and NMR). AKTA Pure FPLC enables us to purify 2-3 different proteins at a time using a well-optimized two-step (HisTrap and Size-exclusion columns) purification protocol.
Below are internal rates for UIC, University of Chicago, and Northwestern University. External academic and commercial rates for instruments and staff times are 1.599X and 2X, respectively. Consumables are same for all users.
Please note that rates are subject to change.
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Protein over-expression and Purification
Service Name Estimated UIC internal price Unit Transformation into BL21DE3 or Rosetta2DE3 $75.00 1 plasmid Prepare glycerol stock $25.00 1 plasmid Protein Mini-expression test $180.00 1 protein, $70/additional sample 1-2L over-expression $200.00 1 protein Purification_Cell lysis-Affinity (HisTrap, or GSTtrap) $700.00 1 protein Purification_SEC (16/60 SD75 or SD200 or 26/60 SD200) $239.00 for 16/60 SD75 or SD200 1 protein Tag cleavage & reload $200.00 1 protein Purification_FPLC run_Affinity service $258.30 1 protein Purification_FPLC run_Ion Exchange Service $270.30 1 protein
Trainings Heading link
Below are internal rates for UIC, University of Chicago, and Northwestern University. External academic and commercial rates are 1.599X and 2X, respectively.
Please note that rates are subject to change.
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Trainings
Instrument Name 1 person 2 people in a group 3 people in a group Unit SPR BiacoreT200 or 8K for new users $329.27 $230.49 $181.10 1 person SPR Biacore 8K for T200 users $209.79 $146.85 $115.38 1 person Integra VIAFLO384 Pipette Head $27.30 $20.48 $16.68 1 person Refeyn TwoMP Mass Photometer $165.00 $110.00 $91.67 1 person Jasco 815 Circular Dichroism (CD) $91.68 $64.18 $49.62 1 person PEAQ-ITC MicroCalorimeter $165.00 $110.00 $91.67 1 person VP-ITC MicroCalorimeter $240.60 $168.42 $132.33 1 person FPLC_AKTA Pure $255.60 $178.92 $140.58 1 person NanoDrop Micro-UV/Vis Spectrophotomenter $12.63 $8.84 $6.95 1 person C25KC Incubator shaker $9.23 $6.46 $5.08 1 person Inova 4200 Incubator shaker $9.23 $6.46 $5.08 1 person Centrifuge_Sorvall LYNX 4000 $14.03 $9.82 $7.72 1 person Emulsiflex C5 Homogenizer $90.22 $63.16 $49.62 1 person Sonic Dismembrator Model500 Sonicator $60.15 $42.10 $33.08 1 person Fisher Scientific 60 Sonic Dismembrator $60.15 $42.10 $33.08 1 person PerkinElmer Victor3V plate reader $57.65 $40.35 $31.71 1 person
Macromolecular Structural Biology Heading link
Biophysics core will assist research projects needing macromolecular structure determination and the biophysical characterization of proteins in collaboration using X-ray crystallography (at Argonne) and Cryo-EM (at University of Chicago). Please contact Hyun Lee (danielhl@uic.edu).